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Targeted nucleotide editing using hybrid prokaryotic and vertebrate adaptive immune systems.
Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
Science. 2016 Aug 4. pii: aaf8729.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
79612pRS315e_pGal-PmCDA1Expresses PmCDA1 in yeast cells
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79613pRS315e_pGal-dCas9-SH3Expresses dCas9-SH3 in yeast cells
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79614pRS315e_pGal-dCas9-SH3-pGal-PmCDA1-SHLExpresses dCas9-SH3 and PmCDA1-SHL in yeast cells
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79615pRS315e_pGal-dCas9-PmCDA1Expresses dCas9-PmCDA1 in yeast cells
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79616pRS415_pGal-nCas9(H840A)Expresses nCas9(H840A) in yeast cells
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79617pRS315e_pGal-nCas9(D10A)-PmCDA1Expresses nCas9(D10A)-PmCDA1 in yeast cells
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79618pRS415_pGal-nCas9(D10A)Expresses nCas9(D10A) in yeast cells
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79619pcDNA3.1_pCMV-nCas-PmCDA1 pH1-gRNA(HPRT)Expresses nCas9-PmCDA1 and gRNA(HPRT) in mammalian cells
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79620pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells
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79621pcDNA3.1_pCMV-dCas-PmCDA1 pH1-gRNA(HPRT)Expresses dCas9-PmCDA1 and gRNA(HPRT) in mammalian cells
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