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Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease.
Nekrasov V, Staskawicz B, Weigel D, Jones JD, Kamoun S
Nat Biotechnol. 2013 Aug 8;31(8):691-3. doi: 10.1038/nbt.2655.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
46965pK7WGF2::hCas9Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue
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46966pICH86966::AtU6p::sgRNA_PDSExpresses an sgRNA targeting the PDS gene in Nicotiana benthamiana from the Arabidopsis U6 promoter
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46968pICSL01009::AtU6pEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.
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