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Human Interferon-Stimulated Gene CRISPR Knockout Library
(Pooled Library #125753)

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 125753 gRNA pooled library 1 $400 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    1,902
  • gRNAs
    15,416
  • Controls
    200
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

This Human Interferon-Stimulated Gene library was developed in collaboration with the Overbaugh and Paddison labs at Fred Hutch.

This ISG-targeting CRISPR KO library was used in Roesch et al (2018) to screen for ISGs that inhibit VSV/G-pseudotyped lentiviral particles. The curation of the list of ISGs and the library design and synthesis are described in Ohainle et al (2018). The same pooled sgRNAs have also been cloned into an HIV-specific CRISPR knockout plasmid, the HIV-CRISPR vector, to create an HIV-specific CRISPR KO library, called the PIKA-HIV (Packageable ISG Knockout Assembly). This CRISPR KO library specific for HIV research is available through the NIH AIDS Reagent Program (Catalog #13566).

The depositing lab recommends using MAGeCK algorithm for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lentiviral Interferon Stimulated Gene Library was a gift from Michael Emerman and Molly OhAinle (Addgene #125753)
  • For your References section:

    A CRISPR screen for factors regulating SAMHD1 degradation identifies IFITMs as potent inhibitors of lentiviral particle delivery. Roesch F, OhAinle M, Emerman M Retrovirology. 2018 Mar 20;15(1):26. doi: 10.1186/s12977-018-0409-2. PubMed 29554922
    A virus-packageable CRISPR screen identifies host factors mediating interferon inhibition of HIV. OhAinle M, Helms L, Vermeire J, Roesch F, Humes D, Basom R, Delrow JJ, Overbaugh J, Emerman M eLife. 2018 Dec 6;7:e39823. doi: 10.7554/eLife.39823. PubMed 30520725