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His-NavMs
(Plasmid #100004)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 100004 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 100004-D50 50 μg of DNA in Tris buffer $495
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DNA 100004-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pTracer-CMV2, IRES GFP
  • Backbone manufacturer
    Thermofisher
  • Backbone size w/o insert (bp) 6200
  • Total vector size (bp) 7100
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    His-NavMs, Voltage gated sodium channel
  • Species
    Magnetococcus marinus
  • Insert Size (bp)
    882
  • Mutation
    C-terminus from a codon-optimized synthetic gene, starting at residue H237
  • GenBank ID
    CP000471.1
  • Promoter CMV
  • Tag / Fusion Protein
    • Hexa-His tag followed by a thrombin cleavage site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer Unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Note: The GFP variant in this plasmid is missing the last five amino acids compared to the closest reference sequence (AAC53663.1). The depositor has confirmed that this does not impact plasmid function.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    His-NavMs was a gift from Paul DeCaen (Addgene plasmid # 100004 ; http://n2t.net/addgene:100004 ; RRID:Addgene_100004)

  • For your References section:

    The complete structure of an activated open sodium channel. Sula A, Booker J, Ng LC, Naylor CE, DeCaen PG, Wallace BA. Nat Commun. 2017 Feb 16;8:14205. doi: 10.1038/ncomms14205. 10.1038/ncomms14205 PubMed 28205548
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