pJO629
(Plasmid #104342)

• Purpose: Express yeast Yap5-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
• Depositing Lab: Alexander Varshavsky
• Publication: Oh et al J Biol Chem. 2017 Dec 29;292(52):21457-21465. doi: 10.1074/jbc.M117.814236. Epub 2017 Nov 9.

Backbone

• Vector backbone: pRS313
• Backbone size w/o insert (bp): 4967
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: pTDH3tc3-Yap5-3xFLAG-tCyc1-pTDHtc3-flagDHFRha-tCyc1
• Alt name: PRT (promoter reference technique)
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 3788
• Entrez Gene: YAP5 (a.k.a. YIR018W)
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13-forward
• 3′ sequencing primer: M13-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The 3788 bp insert covers entire PRT module.For cloning of gene of interest, just use Asc I/Bam HI sites.