pG-HIV-SIN-2LTR
(Plasmid #104591)

• Purpose: Standard for qPCR
• Depositing Lab: Kristine Yoder
• Publication: Yoder et al J Virol Methods. 2008 Nov;153(2):253-6. doi: 10.1016/j.jviromet.2008.07.032. Epub 2008 Sep 17.

Backbone

• Vector backbone: pGemT
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 3001
• Vector type: cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 2LTR circle junction of self-inactivating HIV-based vectors
• Species: HIV-1 based vector

Cloning Information

• Cloning method: TOPO Cloning
• 5′ sequencing primer: T7-F TAATACGACTCACTATAGGG and SP6-F ATTTAGGTGACACTATAG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The self-inactivating (SIN) HIV-1 vector has sequence from the LTR deleted, including the sequence encoded by qPCR primer MH536. An alternative primer set and qPCR standard plasmid was made to detect HIV-1 SIN vector 2LTR circles. The 2LTR circle insert is Addgene 753-929 bp. It may be amplified with primers MH535 (5' AACTAGGGAACCCACTGCTTAAG), KY268 (5' CCAGAGAGACCCAGTACAAGCA), and Taqman probe MH603 (5' ACACTACTTGAAGCACTCAAGGCAAGCTTT)