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ephrinB3Fc #RP23
(Plasmid #104905)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104905 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pIgBOS/PEF BOS
  • Total vector size (bp) 7579
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EphrinB3
  • Species
    H. sapiens (human)
  • Mutation
    contains aa 28-223 of EphrinB3
  • Promoter EF1a
  • Tags / Fusion Proteins
    • mIL3 signal peptide 1-32 (N terminal on insert)
    • FLAG (N terminal on insert)
    • Gly6-TEV-hIgG1Fc (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site BamHI (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Depositor Comments

map of plasmid contained in the pdf detailing cloning

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ephrinB3Fc #RP23 was a gift from Andrew Boyd (Addgene plasmid # 104905)
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