ephrinB3Fc #RP23
(Plasmid #105062)

• Purpose: EphB3 extracellular domain fused to human IgG1 Fc for protein production
• Depositing Lab: Andrew Boyd
• Depositing Organization: QIMR Berghofer Medical Research Institute
  Located in Australia
• Publication: Boyd Lab Ephrin-Fc plasmids (unpublished)

Backbone

• Vector backbone: pIgBOS/PEF BOS
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ephrin B3
• Alt name: NM_001406.3
• Species: H. sapiens (human)
• Mutation: contains aa 28-223
• Promoter: EF1alpha
• Tags / Fusion Proteins:
  • IL3 signal peptide, Flag tag (N terminal on backbone)
  • Gly spacer, TEV, human IgG1 Fc (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The pTIg-BOS-Fc vector retains the advantages of the Ig fusion protein: easy detection using anti-human IgG1 antibodies and rapid purification by protein A Sepharose affinity chromatography. Furthermore, by cleaving the fusion protein with TEV protease, the single chain recombinant target protein can be separated from the IgG-Fc for functional studies.