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pcDNA5/FRT/TO 3xFlag mRuby BuR1 528 (3A mut) FSIFDE->FSAADA (wobbled D9) (#3092)
(Plasmid #106679)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106679 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BuR1 528 (3A mut) FSIFDE->FSAADA (wobbled D9)
  • Species
    H. sapiens (human)
  • Mutation
    528 (3A mut) FSIFDE->FSAADA
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag mRuby (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA5/FRT/TO 3xFlag mRuby BuR1 528 (3A mut) FSIFDE->FSAADA (wobbled D9) (#3092) was a gift from Jonathon Pines (Addgene plasmid # 106679)

  • For your References section:

    The Mitotic Checkpoint Complex Requires an Evolutionary Conserved Cassette to Bind and Inhibit Active APC/C. Di Fiore B, Wurzenberger C, Davey NE, Pines J. Mol Cell. 2016 Dec 15;64(6):1144-1153. doi: 10.1016/j.molcel.2016.11.006. Epub 2016 Dec 8. 10.1016/j.molcel.2016.11.006 PubMed 27939943
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