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pLenti sgRNA-Gfi1-U6-sgRNA-Pou4f3 / MS2-P65-HSF1 mScarlet
(Plasmid #106931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 106931 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pLenti
  • Total vector size (bp) 12293
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    MS2
  • Species
    Synthetic
  • Tags / Fusion Proteins
    • p65 (C terminal on insert)
    • HSF1 (C terminal on insert)
    • 2A skipping peptide (C terminal on insert)
    • mScarlet (C terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    Truncated sgRNA targeting murine Gfi1 (isoform 1) promoter
  • Species
    M. musculus (mouse), Synthetic
  • Mutation
    Contains two MS2 aptamer hairpins

Gene/Insert 3

  • Gene/Insert name
    Truncated sgRNA targeting murine Pou4f3 promoter
  • Species
    M. musculus (mouse), Synthetic
  • Mutation
    Contains two MS2 aptamer hairpins

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti sgRNA-Gfi1-U6-sgRNA-Pou4f3 / MS2-P65-HSF1 mScarlet was a gift from Albert Edge (Addgene plasmid # 106931)
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