pcDNA5-GFP-PKHD1
(Plasmid #108408)

• Purpose: Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.
• Depositing Lab: Gregory Germino
• Depositing Organization: Johns Hopkins University and School of Medicine
  Located in United States of America
• Publication: Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56.

Backbone

• Vector backbone: pcDNA5
• Backbone manufacturer: Invitrogen
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PKHD1
• Alt name: fibrocystin/polyductin
• Alt name: FPC
• Species: H. sapiens (human)
• Entrez Gene: PKHD1 (a.k.a. ARPKD, FCYT, FPC, PCYT, PKD4, TIGM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • eGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene sequencing results find V1463A, L1870V, S3864P, and Q3899R mutations in PKHD1 compared to the NCBI reference sequence NM_138694.3. The S3864P mutation is specific to this plasmid from the publication. It is unknown how these mutations affect plasmid function.

DNA (Catalog # 108408-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 108408-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.