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(Plasmid #110726)


Item Catalog # Description Quantity Price (USD)
Plasmid 110726 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 8102
  • Total vector size (bp) 13371
  • Modifications to backbone
    None other than cloning of insert
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human), Synthetic
  • Mutation
    See attached PDF
  • GenBank ID
    AAB94054.3 P02452.5
  • Entrez Gene
    COL1A1 (a.k.a. CAFYD, EDSARTH1, EDSC, OI1, OI2, OI3, OI4)
  • Promoter CMV
  • Tags / Fusion Proteins
    • mGFP
    • SBP: streptavidin binding peptide

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CGC AAA TGG GCG GTA GGC GTG
  • 3′ sequencing primer GATGGCCACATCGGCAG
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See attached PDF for full descritions

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLVX-SBP-mGFP-COL1A1 was a gift from David Stephens (Addgene plasmid # 110726)
  • For your References section:

    ER-to-Golgi trafficking of procollagen in the absence of large carriers. McCaughey J, Stevenson NL, Cross S, Stephens DJ. J Cell Biol. 2018 Dec 26. pii: jcb.201806035. doi: 10.1083/jcb.201806035. 10.1083/jcb.201806035 PubMed 30587510