pLG_GI4
(Plasmid
#111595)
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PurposeGI Library Vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 111595 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSICO derivative
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 8200
- Total vector size (bp) 8888
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namesgRNA
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gRNA/shRNA sequenceGNNNNNNNNNNNNNNNNNNN
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLG_GI4 was a gift from Jonathan Weissman (Addgene plasmid # 111595 ; http://n2t.net/addgene:111595 ; RRID:Addgene_111595) -
For your References section:
Mapping the Genetic Landscape of Human Cells. Horlbeck MA, Xu A, Wang M, Bennett NK, Park CY, Bogdanoff D, Adamson B, Chow ED, Kampmann M, Peterson TR, Nakamura K, Fischbach MA, Weissman JS, Gilbert LA. Cell. 2018 Aug 9;174(4):953-967.e22. doi: 10.1016/j.cell.2018.06.010. Epub 2018 Jul 19. 10.1016/j.cell.2018.06.010 PubMed 30033366
