PurposeHigh copy number plasmid containing Ple320 (POU4F1 MiniPromoter) insert.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||111888||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)1969
Entrez GenePOU4F1 (a.k.a. BRN3A, Oct-T1, RDC-1, brn-3A)
- Promoter Ple320 (POU4F1 MiniPromoter)
- Cloning method Unknown
- 5′ sequencing primer M13-fwd
- 3′ sequencing primer M13-rev (Common Sequencing Primers)
Plasmid commercially produced by Genscript . Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid, including the MiniPromoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEMS2235 was a gift from Elizabeth Simpson (Addgene plasmid # 111888 ; http://n2t.net/addgene:111888 ; RRID:Addgene_111888)
For your References section:New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression in Retinal Ganglion Cells of Mouse and Primate Retina. Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW. Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118. 10.1089/hum.2018.118 PubMed 30062914