pNB2629
(Plasmid
#113394)
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PurposeMET25p-MS2-RFP-CYC1 TT
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 113394 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS315
- Total vector size (bp) 7620
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameMS2 bacteriophage coat protein
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Alt nameMS2
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SpeciesS. cerevisiae (budding yeast)
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Tag
/ Fusion Protein
- red fluorescent protein (RFP), codon-optimized for S. cerevisiae (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNB2629 was a gift from Suzanne Sandmeyer (Addgene plasmid # 113394 ; http://n2t.net/addgene:113394 ; RRID:Addgene_113394) -
For your References section:
Sequence requirements for localization and packaging of Ty3 retroelement RNA. Clemens K, Bilanchone V, Beliakova-Bethell N, Larsen LS, Nguyen K, Sandmeyer S. Virus Res. 2013 Feb;171(2):319-31. doi: 10.1016/j.virusres.2012.10.008. Epub 2012 Oct 13. 10.1016/j.virusres.2012.10.008 PubMed 23073180
