Skip to main content
Addgene
Open Menu

pCMV-myc-Sema4A
(Plasmid #115171)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115171 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 115171-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 115171-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pCMV
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Sema4A
  • Alt name
    Semaphorin 4A
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    2200
  • Mutation
    First 32 amino acids of Sema4A replaced with membrane targeting sequence
  • GenBank ID
  • Entrez Gene
    Sema4a (a.k.a. AI132332, SemB, Semab)
  • Promoter CMV
  • Tags / Fusion Proteins
    • membrane targeting sequence (N terminal on backbone)
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcttatggccatggaggcccgaACTGGTGGTCAGGGGCCC
  • 3′ sequencing primer tcgagagatctcggtcgaccgTCAAGCCACTTCGGCGCC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-myc-Sema4A was a gift from Suzanne Paradis (Addgene plasmid # 115171 ; http://n2t.net/addgene:115171 ; RRID:Addgene_115171)

  • For your References section:

    Class 4 Semaphorins and Plexin-B receptors regulate GABAergic and glutamatergic synapse development in the mammalian hippocampus. McDermott JE, Goldblatt D, Paradis S. Mol Cell Neurosci. 2018 Jul 4;92:50-66. doi: 10.1016/j.mcn.2018.06.008. 10.1016/j.mcn.2018.06.008 PubMed 29981480
Related items:
Commonly requested with: