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pBW2707 (Myr v-RasH delta 766)
(Plasmid #115312)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 115312 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pGV16
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    v-Ras H
  • Species
    R. norvegicus (rat)
  • Mutation
    1-165 PDQ 184-189. N-term 1-165 and C-term 184-189 amino acids joined by an oligonucleotide linker encoding the the three amino acids indicated (amino acids 166-183 deleted)
  • Entrez Gene
    Hras (a.k.a. HRAS1, c-H-ras)
  • Tag / Fusion Protein
    • 15 aa myristoylation sequence derived from src (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBW2707 (Myr v-RasH delta 766) was a gift from Berthe Willumsen (Addgene plasmid # 115312)

  • For your References section:

    Novel determinants of H-Ras plasma membrane localization and transformation. Willumsen BM, Cox AD, Solski PA, Der CJ, Buss JE. Oncogene. 1996 Nov 7;13(9):1901-9. PubMed 8934536
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