Depositor Sequences: None.
Addgene Sequences: None. Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||11536||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6143
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameFAD synthetase
Insert Size (bp)882
GenBank IDAAB96154 MPN330 NP_110018
/ Fusion Protein
- HisTag, non-cleavable (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer TAATACGACTCACTATAGGG
- 3′ sequencing primer GTTGCATCACCTTCACCCTCTCC (Common Sequencing Primers)
Terms and Licenses
Please note that the plasmid pLIC.B2.MPN330 contains an Ampicillin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons.
Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLIC.B2.MPN330 was a gift from Sung-Hou Kim (Addgene plasmid # 11536)
For your References section:A model for the determination of the 3D-spatial distribution of the functions of the hormone-binding domain of receptors that bind 3-keto-4-ene steroids. Lemesle-Varloot L, Ojasoo T, Mornon JP, Raynaud JP. J Steroid Biochem Mol Biol. 1992 Mar . 41(3-8):369-88. 10.1016/0960-0760(92)90363-N PubMed 1562512
Generated by Addgene from plasmid data.