pBS314 EIIa-Cre
(Plasmid #11917)

• Depositing Lab: Brian Sauer
• Depositing Organization: National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK)
  Located in the United States of America
• Publication: Lakso et al Proc Natl Acad Sci U S A. 1996 Jun 11. 93(12):5860-5.

Backbone

• Vector backbone: n/a
• Backbone size w/o insert (bp): 6200
• Vector type: Mammalian Expression, Cre/Lox

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: cre
• Species: bacteriophage P1
• Insert Size (bp): 1100
• Entrez Gene: cre (a.k.a. P1_gp003)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBS314 is the parental plasmid from which the EIIa-cre transgene construct was excised for microinjection into fertilized zygotes to produce EIIa-cre mice. In these mice Cre is expressed in fertilized zygotes and preimplantation embryos, but not subsequently. Such mice have been used either to delete a loxP-flanked transgene or to turn on expression of a transgene by recombinational activation of gene expression. The temporal limitation of Cre expression and recombination to early embryogenesis also provides a convenient mechanism to generate mosaic mice for targeted gene ablation or gene activation.

EIIa-cre mice are available from the Jackson Laboratory.

For more information, visit http://www.geocities.com/[email protected]/pBS314.html