FB026
(Plasmid #119711)

• Purpose: Transcriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMT
• Depositing Lab: Jose Marcos
• Publication: Hernanz-Koers et al Fungal Genet Biol. 2018 Jul;116:51-61. doi: 10.1016/j.fgb.2018.04.010. Epub 2018 Apr 20.

Backbone

• Vector backbone: PDGB3alpha1R
• Backbone manufacturer: Diego Orzaez
• Backbone size w/o insert (bp): 6969
• Total vector size (bp): 8089
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Promoter gpdA:YFP coding sequence:Terminator trpC
• Alt name: FB007+GB0053+FB008
• Species: Aspergillus nidulans (Pgpda), A. victoria (yfp), Aspergillus nidulans (TtrpC)
• Insert Size (bp): 1710

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bsa1 (destroyed during cloning)
• 3′ cloning site: Bsa1 (destroyed during cloning)
• 5′ sequencing primer: CGAGTGGTGATTTTGTGCCG
• 3′ sequencing primer: CCCGCCAATATATCCTGTCAG

Resource Information

• Supplemental Documents:
  • 04_FB026 (GB_UA_25A7).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry