AAV CMV-driven AcrIIA4-2xmiR-122 target sites
PurposeAAV vector for expression of AcrIIA4 with two miR-122 binding sites
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||120298||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, AAV, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert nameAcrIIA4-2xmiR-122 binding sites
Alt nameU6 scaffold
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
Please visit https://www.biorxiv.org/content/10.1101/480384v1 for BioRxiv preprint
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:AAV CMV-driven AcrIIA4-2xmiR-122 target sites was a gift from Dominik Niopek (Addgene plasmid # 120298 ; http://n2t.net/addgene:120298 ; RRID:Addgene_120298)
For your References section:Cell-specific CRISPR-Cas9 activation by microRNA-dependent expression of anti-CRISPR proteins. Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri J, Mastel M, Borner K, Eils R, Grimm D, Niopek D. Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271. 10.1093/nar/gkz271 PubMed 30982889