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pJS1a (tsE2)
(Plasmid #12138)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 12138 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 12138-D50 50 μg of DNA in Tris buffer $495
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DNA 12138-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    C59-451
  • Backbone manufacturer
    D. DiMaio (Yale)
  • Backbone size w/o insert (bp) 2900
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tsE2-neo
  • Alt name
    BPV E2
  • Species
    Bovine papilloma virus
  • Insert Size (bp)
    3000
  • Tag / Fusion Protein
    • neo (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This plasmid contains a promoterless neomycin resistance gene inserted in tandem with the tsE2 gene. The tsE2 gene (DiMaio and Settleman, 1988) is a temperature-sensitive BPV-E2 allele constructed by insertional mutagenesis. The expressed tsE2 protein fails to transactivate
an LCR-SV40 hybrid promoter at 37'C. At 32.5'C the mutant
protein functions like its wild type counterpart to induce gene expression from the hybrid promoter.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJS1a (tsE2) was a gift from James Sherley (Addgene plasmid # 12138 ; http://n2t.net/addgene:12138 ; RRID:Addgene_12138)

  • For your References section:

    Guanine nucleotide biosynthesis is regulated by the cellular p53 concentration. Sherley JL. J Biol Chem. 1991 Dec 25. 266(36):24815-28. PubMed 1761576
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