pJS1a (tsE2)
(Plasmid #12138)

• Depositing Lab: James Sherley
• Publication: Sherley et al J Biol Chem. 1991 Dec 25. 266(36):24815-28.

Backbone

• Vector backbone: C59-451
• Backbone manufacturer: D. DiMaio (Yale)
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tsE2-neo
• Alt name: BPV E2
• Species: Bovine papilloma virus
• Insert Size (bp): 3000
• Tag / Fusion Protein:
  • neo (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: na

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a promoterless neomycin resistance gene inserted in tandem with the tsE2 gene. The tsE2 gene (DiMaio and Settleman, 1988) is a temperature-sensitive BPV-E2 allele constructed by insertional mutagenesis. The expressed tsE2 protein fails to transactivate
an LCR-SV40 hybrid promoter at 37'C. At 32.5'C the mutant
protein functions like its wild type counterpart to induce gene expression from the hybrid promoter.