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pLARRY-EGFP Expressed Barcode Library
(Plasmid #122519)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 122519 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pLV
  • Backbone size w/o insert (bp) 7712
  • Total vector size (bp) 8432
  • Modifications to backbone
    Inverted cassette (EF1a promoter - unidirectional polyA)
  • Vector type
    Lentiviral
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    -
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EGFP
  • Species
    Synthetic
  • Insert Size (bp)
    720
  • Promoter EF1alpha

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer gttaggccagcttggcacttgat
  • 3′ sequencing primer gcacaggtcgacaccagt
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    -

Depositor Comments

-

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLARRY-EGFP Expressed Barcode Library was a gift from (Addgene plasmid # 122519)
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