pGEM-T Easy-Gbait-hs-Cre
(Plasmid
#122562)
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PurposeIntegration of Cre sequence using the CRISPR/Cas9 system.
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Depositing Lab
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Depositing OrganizationCarnegie Institution for Science
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 122562 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 122562-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 122562-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGEM-T Easy
- Total vector size (bp) 4996
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Vector typeCloning
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGbait-hsp70-Cre-SV40pA
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SpeciesD. rerio (zebrafish)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacII (not destroyed)
- 3′ cloning site SacII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was constructed by modifying the Gbait-hsp70:Gal4 donor plasmid described in Kimura et al. “Efficient generation of knock-in transgenic zebrafish carrying reporter/driver genes by CRISPR/Cas9-mediated genome engineering” Scientific reports vol. 4 6545. 8 Oct. 2014, doi:10.1038/srep06545.
DNA (Catalog # 122562-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 122562-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-T Easy-Gbait-hs-Cre was a gift from Marnie Halpern (Addgene plasmid # 122562 ; http://n2t.net/addgene:122562 ; RRID:Addgene_122562) -
For your References section:
Specialized neurons in the right habenula mediate response to aversive olfactory cues. Choi JH, Duboue ER, Macurak M, Chanchu JM, Halpern ME. Elife. 2021 Dec 8;10:e72345. doi: 10.7554/eLife.72345. 10.7554/eLife.72345 PubMed 34878403
Map uploaded by the depositor.
