pmCherry_sgRNA-ver2
(Plasmid
#126776)
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PurposegRNA expression vector with mCherry transfection control, does not contain the direct repeat of the truncated guideRNA
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Depositing Lab
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Depositing OrganizationHUN-REN Research Centre for Natural Sciences
Located in Hungary -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126776 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 126776-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 126776-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmCherry
- Total vector size (bp) 4955
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Vector typeMammalian Expression, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgRNA (for SpCas9)
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gRNA/shRNA sequencegRNA cloning vector
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SpeciesS. pyogenes
- Promoter U6
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CCTCTGACTTGAGCGTCG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
gRNA cloning vector (U6_BbsI sites_sgRNA) with mCherry transfection control (CMV_mCherry cassette), lacks the direct repeat (a truncated guideRNA) after the 3'-end of the sgRNA.
For detailed information and plasmid usage, please see the associated publication.
DNA (Catalog # 126776-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 126776-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmCherry_sgRNA-ver2 was a gift from Ervin Welker (Addgene plasmid # 126776 ; http://n2t.net/addgene:126776 ; RRID:Addgene_126776) -
For your References section:
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs. Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E. Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5. 10.1038/s41467-020-15021-5 PubMed 32144253
Map uploaded by the depositor.
