pET28a-StrepII-sfGFP-TEV-Rab45wt_dtail-His6-FLAG
(Plasmid
#127927)
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PurposeExpress wildtype Rab45 in bacteria
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127927 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepET28a
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pET28a-StrepII-sfGFP-TEV-Rab45wt_dtail-His6-FLAG was a gift from Ron Vale (Addgene plasmid # 127927) -
For your References section:
CRACR2a is a calcium-activated dynein adaptor protein that regulates endocytic traffic. Wang Y, Huynh W, Skokan TD, Lu W, Weiss A, Vale RD. J Cell Biol. 2019 May 6;218(5):1619-1633. doi: 10.1083/jcb.201806097. Epub 2019 Feb 27. 10.1083/jcb.201806097 PubMed 30814157