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pET28a-His-CRACR2awt_dtail-sfGFP-StrepII
(Plasmid #127931)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 127931 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pET28a
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRACR2a
  • Species
    H. sapiens (human)
  • Mutation
    residue 1-708
  • Entrez Gene
    CRACR2A (a.k.a. EFCAB4B, RAB46)
  • Promoter T7
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • sfGFP-StrepII (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28a-His-CRACR2awt_dtail-sfGFP-StrepII was a gift from Ron Vale (Addgene plasmid # 127931)

  • For your References section:

    CRACR2a is a calcium-activated dynein adaptor protein that regulates endocytic traffic. Wang Y, Huynh W, Skokan TD, Lu W, Weiss A, Vale RD. J Cell Biol. 2019 May 6;218(5):1619-1633. doi: 10.1083/jcb.201806097. Epub 2019 Feb 27. 10.1083/jcb.201806097 PubMed 30814157
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