mcherry-rab45-dct-fkbp
(Plasmid
#127953)
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Purposeexpress Rab45 fused to FKBP at the C terminus
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 127953 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepmCherry
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Backbone manufacturern/a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRab45
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Alt nameRasef
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SpeciesH. sapiens (human)
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Entrez GeneRASEF (a.k.a. RAB45, TSG)
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Tags
/ Fusion Proteins
- mCherry (N terminal on insert)
- FKBP (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mcherry-rab45-dct-fkbp was a gift from Ron Vale (Addgene plasmid # 127953) -
For your References section:
CRACR2a is a calcium-activated dynein adaptor protein that regulates endocytic traffic. Wang Y, Huynh W, Skokan TD, Lu W, Weiss A, Vale RD. J Cell Biol. 2019 May 6;218(5):1619-1633. doi: 10.1083/jcb.201806097. Epub 2019 Feb 27. 10.1083/jcb.201806097 PubMed 30814157