pDONRG_P4-P1R:TBA2p
(Plasmid #128429)

• Purpose: Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.
• Depositing Lab: George Haughn
• Publication: McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 10.1007/s11103-019-00909-8.

Backbone

• Vector backbone: pDONRG_P4-P1R
• Backbone manufacturer: Invitrogen modified by doi:10.1271/bbb.70678
• Backbone size w/o insert (bp): 5892
• Total vector size (bp): 5104
• Modifications to backbone: 151 bp within the attR1 sequence there is a thymine to cysteine modification which does not impact the gateway reaction.
• Vector type: Gateway promoter entry clone

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For selection use 7 ug/ml Gentamicin
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TESTA ABUNDANT 2
• Alt name: TBA2
• Alt name: AT1G62060.1
• Alt name: O04575 (Uniprot)
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1346
• GenBank ID: AY125570.1 NCBI GeneID: 842502
• Entrez Gene: AT1G62060 (a.k.a. AT1G62060, F19K23.1, F19K23_1)
• Promoter: TESTA ABUNDANT 2

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: M13F: 5’-TGTAAAACGACGGCCAGT-3’
• 3′ sequencing primer: M13R-pUC: 5’-CAGGAAACAGCTATGAC-3’

Resource Information

• Supplemental Documents:
  • pDONRG_P4-P1R-TBA2p-Plasmid Map - SnapGene File

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Industry MTA

Depositor Comments

For use with Three-way Multi-site Gateway Cloning (Invitrogen).