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PurposeVisualization of Akt and ERK activity
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Depositing Lab
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Depositing OrganizationUniversity of Amsterdam
Located in Netherlands -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129632 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 129632-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 129632-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePiggyBac
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2021.07.27.453948v3 for bioRxiv preprint.
DNA (Catalog # 129632-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 129632-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-H2A-mScarlet-2A-AKT_KTR-mTq2-2A-ERK_KTR-mNG was a gift from Dorus Gadella (Addgene plasmid # 129632 ; http://n2t.net/addgene:129632 ; RRID:Addgene_129632) -
For your References section:
Single-cell imaging of ERK and Akt activation dynamics and heterogeneity induced by G-protein-coupled receptors. Chavez-Abiega S, Gronloh MLB, Gadella TWJ, Bruggeman FJ, Goedhart J. J Cell Sci. 2022 Mar 15;135(6):jcs259685. doi: 10.1242/jcs.259685. Epub 2022 Mar 11. 10.1242/jcs.259685 PubMed 35107584
