Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pX458M-53BP1-DN1S
(Plasmid #131045)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 131045 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    PX458M
  • Backbone size w/o insert (bp) 9299
  • Total vector size (bp) 10583
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SpCas9-53BP1-DN1S
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1284
  • Mutation
    Only expressing residues 1231-1644 of complete 53BP1 CDS.
  • GenBank ID
    NM_005657.2
  • Entrez Gene
    TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
  • Promoter CBH
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • SpCas9 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SanDI (not destroyed)
  • 3′ cloning site FseI (not destroyed)
  • 5′ sequencing primer GAT CAC AAA GCA CGT GGC ACA G
  • 3′ sequencing primer GAA CAG CTC CTC GCC CTT GC
  • (Common Sequencing Primers)

Terms and Licenses

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX458M-53BP1-DN1S was a gift from Punam Malik (Addgene plasmid # 131045 ; http://n2t.net/addgene:131045 ; RRID:Addgene_131045)
  • For your References section:

    CRISPR-Cas9 fusion to dominant-negative 53BP1 enhances HDR and inhibits NHEJ specifically at Cas9 target sites. Jayavaradhan R, Pillis DM, Goodman M, Zhang F, Zhang Y, Andreassen PR, Malik P. Nat Commun. 2019 Jun 28;10(1):2866. doi: 10.1038/s41467-019-10735-7. 10.1038/s41467-019-10735-7 PubMed 31253785