pX458M-53BP1-DN1S
(Plasmid #131045)

• Purpose: Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.
• Depositing Lab: Punam Malik
• Publication: Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi: 10.1038/s41467-019-10735-7.

Backbone

• Vector backbone: PX458M
• Backbone size w/o insert (bp): 9299
• Total vector size (bp): 10583
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SpCas9-53BP1-DN1S
• Species: H. sapiens (human)
• Insert Size (bp): 1284
• Mutation: Only expressing residues 1231-1644 of complete 53BP1 CDS.
• GenBank ID: NM_005657.2
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Promoter: CBH
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • SpCas9 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SanDI (not destroyed)
• 3′ cloning site: FseI (not destroyed)
• 5′ sequencing primer: GAT CAC AAA GCA CGT GGC ACA G
• 3′ sequencing primer: GAA CAG CTC CTC GCC CTT GC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry