pSP72 syn-CTAP AphAI
(Plasmid
#133954)
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Purpose(Empty Backbone) Vector for BAC mutagenesis via Red recombination (kanR excised upon I-SceI and Rec E/T induction) with disrupted affinity tag (synthetic ORF eliminates nucleotide repeats present in wild-type form); kanamycin resistance; restriction enzyme cloning.
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133954 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepSP72 syn-CTAP AphAI
- Backbone size (bp) 3968
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
HMS clone ID number: EvNO00025963
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSP72 syn-CTAP AphAI was a gift from Donald Coen (Addgene plasmid # 133954) -
For your References section:
A generic protein purification method for protein complex characterization and proteome exploration. Rigaut G, Shevchenko A, Rutz B, Wilm M, Mann M, Séraphin B. Nat Biotechnol. 1999 Oct . 17(10):1030-2. 10.1038/13732 PubMed 10504710
Map uploaded by the depositor.
