pTVKRSF225
(Plasmid
#135005)
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PurposeMusF2 prenyltransferases from Nmu. PCC 7906
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135005 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRSF
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMusF2 prenyltransferaseM
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SpeciesNostoc sp. PCC 7906
- Promoter T7
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Tag
/ Fusion Protein
- SUMO fusion (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer SUMO fw
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
It was published as Addgene ID # 135005
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTVKRSF225 was a gift from Hideo Iwai (Addgene plasmid # 135005 ; http://n2t.net/addgene:135005 ; RRID:Addgene_135005) -
For your References section:
Biosynthesis of the Bis-Prenylated Alkaloids Muscoride A and B. Mattila A, Andsten RM, Jumppanen M, Assante M, Jokela J, Wahlsten M, Mikula KM, Sigindere C, Kwak DH, Gugger M, Koskela H, Sivonen K, Liu X, Yli-Kauhaluoma J, Iwai H, Fewer DP. ACS Chem Biol. 2019 Dec 20;14(12):2683-2690. doi: 10.1021/acschembio.9b00620. Epub 2019 Nov 19. 10.1021/acschembio.9b00620 PubMed 31674754
