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Addgene

pJES103
(Plasmid #135410)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 135410 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pmv261
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    blaTEM-1
  • Species
    E. coli
  • Mutation
    E. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence
  • Promoter hsp60

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJES103 was a gift from Miriam Braunstein (Addgene plasmid # 135410 ; http://n2t.net/addgene:135410 ; RRID:Addgene_135410)
  • For your References section:

    Beta-lactamase can function as a reporter of bacterial protein export during Mycobacterium tuberculosis infection of host cells. McCann JR, McDonough JA, Pavelka MS, Braunstein M. Microbiology. 2007 Oct;153(Pt 10):3350-9. doi: 10.1099/mic.0.2007/008516-0. 10.1099/mic.0.2007/008516-0 PubMed 17906134
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