pJES103
(Plasmid
#135410)
-
PurposeE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence under the control of hsp60 promoter that can be used as a reporter
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 135410 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepmv261
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameblaTEM-1
-
SpeciesE. coli
-
MutationE. coli 'blaTEM-1 with M. tuberculosis mpt63 signal sequence
- Promoter hsp60
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pJES103 was a gift from Miriam Braunstein (Addgene plasmid # 135410 ; http://n2t.net/addgene:135410 ; RRID:Addgene_135410) -
For your References section:
Beta-lactamase can function as a reporter of bacterial protein export during Mycobacterium tuberculosis infection of host cells. McCann JR, McDonough JA, Pavelka MS, Braunstein M. Microbiology. 2007 Oct;153(Pt 10):3350-9. doi: 10.1099/mic.0.2007/008516-0. 10.1099/mic.0.2007/008516-0 PubMed 17906134