pMal-EcPhr
(Plasmid #136244)

• Purpose: Protein expression of E. coli Phr
• Depositing Lab: Aziz Sancar
• Publication: Selby et al Proc Natl Acad Sci U S A. 2006 Nov 21. 103(47):17696-700.

Backbone

• Vector backbone: pMalc2
• Backbone manufacturer: NEB
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EcPhr
• Species: E. coli
• Mutation: V115E- Please see depositor comments below
• Tag / Fusion Protein:
  • MBP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Depositor confirms V115E mutation in EcPhr does not affect plasmid function.