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pCDH-EF1-SNAP-mTurquoise2-H2A-IRES-Puro
(Plasmid #138968)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 138968 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pCDH-EF1-MCS-IRES-Puro
  • Backbone manufacturer
    System Bioscience
  • Backbone size w/o insert (bp) 7650
  • Total vector size (bp) 9380
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Histone H2A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1278
  • Promoter EF-1α
  • Tags / Fusion Proteins
    • SNAP-tag (C terminal on backbone)
    • mTurquoise (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CAAGCTGTGACCGGCG
  • 3′ sequencing primer GCTTCGGCCAGTAACGTTA
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

SNAPSwitch: A Molecular Sensor to Quantify the Localization of Proteins, DNA and Nanoparticles in Cells
Laura I FitzGerald, Luigi Aurelio, Moore Chen, Daniel Yuen, Bim Graham, Angus P R Johnston
bioRxiv 2019.12.16.877043; doi: https://doi.org/10.1101/2019.12.16.877043

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH-EF1-SNAP-mTurquoise2-H2A-IRES-Puro was a gift from Angus Johnston (Addgene plasmid # 138968)

  • For your References section:

    SNAPSwitch: A Molecular Sensor to Quantify the Localization of Proteins, DNA and Nanoparticles in Cells. FitzGerald LI, Aurelio L, Chen M, Yuen D, Graham B, Johnston APR. bioRxiv 10.1101/2019.12.16.877043
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