pCS2+-H2B-pr-mEosFP
(Plasmid
#141101)
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PurposeH2B fused to pr-mEosFP for chromatin visualization and primed conversion
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Depositing Lab
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Depositing OrganizationImperial College London
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 141101 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 141101-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 141101-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
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Backbone manufacturerRZPD
- Backbone size w/o insert (bp) 4144
- Total vector size (bp) 5221
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHistone 2B
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Alt nameH2B
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SpeciesH. sapiens (human), M. musculus (mouse), R. norvegicus (rat), D. rerio (zebrafish)
- Promoter simian CMV IE94
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Tag
/ Fusion Protein
- pr-mEosFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (not destroyed)
- 3′ cloning site SnaBI (not destroyed)
- 5′ sequencing primer SP6
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 141101-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 141101-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2+-H2B-pr-mEosFP was a gift from Periklis Pantazis (Addgene plasmid # 141101 ; http://n2t.net/addgene:141101 ; RRID:Addgene_141101) -
For your References section:
Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches. Welling M, Kalyviotis K, Pantazis P. Bio Protoc. 2020 Jun 5;10(11):e3645. doi: 10.21769/BioProtoc.3645. eCollection 2020 Jun 5. 10.21769/BioProtoc.3645 PubMed 33659315
Map uploaded by the depositor.
