Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

(Plasmid #141199)


Item Catalog # Description Quantity Price (USD)
Plasmid 141199 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
    R134H, W309R, Q310A compared to iRFP
  • Promoter T7
  • Tag / Fusion Protein
    • 6x-His (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer ATG CGT CCG GCG TAG A
  • 3′ sequencing primer GAT TAT GCG GCC GTG TAC AA
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    heme oxygenase
  • Insert Size (bp)
  • Promoter T7

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site AvrII (not destroyed)
  • 5′ sequencing primer TTG TAC ACG GCC GCA TAA TC
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains a K193N mutation in mRhubarb713. This mutation is not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-DUET-1-mRhubarb713-HO was a gift from Elad Firnberg (Addgene plasmid # 141199 ; ; RRID:Addgene_141199)
  • For your References section:

    mRhubarb: Engineering of monomeric, red-shifted, and brighter variants of iRFP using structure-guided multi-site mutagenesis. Rogers OC, Johnson DM, Firnberg E. Sci Rep. 2019 Oct 30;9(1):15653. doi: 10.1038/s41598-019-52123-7. 10.1038/s41598-019-52123-7 PubMed 31666599