Skip to main content
Addgene
Open Menu

pFB 5' Flag BRCA1 (1863)
(Plasmid #14507)

Print

Full plasmid sequence is not available for this item.

Loading...

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14507 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BRCA1
  • Species
    H. sapiens (human)
  • Entrez Gene
    BRCA1 (a.k.a. BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53)
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length BRCA1 baculovirus was constructed according to the FastBac system (Invitrogen).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFB 5' Flag BRCA1 (1863) was a gift from Jeffrey Parvin (Addgene plasmid # 14507)

  • For your References section:

    BRCA1-dependent ubiquitination of gamma-tubulin regulates centrosome number. Starita LM, Machida Y, Sankaran S, Elias JE, Griffin K, Schlegel BP, Gygi SP, Parvin JD. Mol Cell Biol. 2004 Oct . 24(19):8457-66. 10.1128/MCB.24.19.8457-8466.2004 PubMed 15367667
Related items: