pAG425 GAL Pong TPase L418A, L420A
(Plasmid
#145795)
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PurposemPing Yeast Transposition Assay
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 145795 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 * | |
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Backbone
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Vector backbonepAG415 GAL
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePong TPase L418A, L420A
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SpeciesOryza Sativa
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MutationL418A, L420A
Cloning Information
- Cloning method Gateway Cloning
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG425 GAL Pong TPase L418A, L420A was a gift from Charles Nathan Hancock & Susan Wessler (Addgene plasmid # 145795 ; http://n2t.net/addgene:145795 ; RRID:Addgene_145795) -
For your References section:
Transposition of the Tourist-MITE mPing in yeast: an assay that retains key features of catalysis by the class 2 PIF/Harbinger superfamily. Hancock CN, Zhang F, Wessler SR. Mob DNA. 2010 Feb 1;1(1):5. doi: 10.1186/1759-8753-1-5. 10.1186/1759-8753-1-5 PubMed 20226077
