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pEF myc Gal cyclin T1 (1-726) (P#1026)
(Plasmid #14626)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 14626 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 14626-D50 50 μg of DNA in Tris buffer $495
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DNA 14626-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pEF-BOS-myc
  • Backbone size w/o insert (bp) 5500
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    cyclin T1
  • Alt name
    cyclinT1
  • Alt name
    cycT1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2186
  • Entrez Gene
    CCNT1 (a.k.a. CCNT, CYCT1, HIVE1)
  • Tags / Fusion Proteins
    • myc (N terminal on backbone)
    • Gal (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer EF1a forward primer
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Full-length cyclin T1 fused to the DBD of Gal4(1–147) and inserted at the BamHI/XbaI sites of pEF-Bos-myc(EF). The cyclin insert contains H519R, S566P, P712S, and L724F compared to closest cyclin T1 reference sequence NP_001231.2.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF myc Gal cyclin T1 (1-726) (P#1026) was a gift from Matija Peterlin (Addgene plasmid # 14626 ; http://n2t.net/addgene:14626 ; RRID:Addgene_14626)

  • For your References section:

    Interaction between P-TEFb and the C-terminal domain of RNA polymerase II activates transcriptional elongation from sites upstream or downstream of target genes. Taube R, Lin X, Irwin D, Fujinaga K, Peterlin BM. Mol Cell Biol. 2002 Jan . 22(1):321-31. 10.1128/MCB.22.1.321-331.2002 PubMed 11739744
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