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pJH1
(Plasmid #154204)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 154204 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCas9
  • Modifications to backbone
    Added an origin of replication from pUCP22; chloramphenicol resistance gene removed and replaced with kanamycin resistance; a portion of the Cas9 protein-encoding region was resynthesized, conserving the encoded amino acid sequence but altering the GC content to 48% (from ~35%); 103bp deleted between the tracrRNA and the Cas9 encoding region.
  • Vector type
    CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Resource Information

  • Supplemental Documents
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJH1 was a gift from Russell Poulter (Addgene plasmid # 154204 ; http://n2t.net/addgene:154204 ; RRID:Addgene_154204)
  • For your References section:

    The application of the CRISPR-Cas9 system in Pseudomonas syringae pv. actinidiae. Ho J, Zhao M, Wojcik S, Taiaroa G, Butler M, Poulter R. J Med Microbiol. 2020 Mar;69(3):478-486. doi: 10.1099/jmm.0.001124. Epub 2020 Jan 14. 10.1099/jmm.0.001124 PubMed 31935181