pCMV-FLAG TIF1gamma M (456-887)
(Plasmid
#15735)
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Depositing Lab
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Depositing OrganizationMemorial Sloan-Kettering Cancer Center
Located in the United States of America -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 15735 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 15735-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 15735-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV5 modified to contain a FLAG epitope
- Backbone size w/o insert (bp) 4600
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTIF1 gamma M
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Alt nameTranscriptional Intermediary Factor 1 gamma
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Alt nameTIF1g
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SpeciesH. sapiens (human)
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Insert Size (bp)1293
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MutationMiddle region (amino acids 456-887).
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Entrez GeneTRIM33 (a.k.a. ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
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Tag
/ Fusion Protein
- Flag (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII/BamHI (destroyed during cloning)
- 3′ cloning site XhoI/SalI (destroyed during cloning)
- 5′ sequencing primer CMV-F
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The indicated coding region was amplified from cDNA templates by polymerase chain reaction (PCR), then subsequently digested with BamHI/XhoI and inserted into the BglII/SalI sites of a modified pCMV5 plasmid containing one copy of Flag epitope.
DNA (Catalog # 15735-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 15735-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-FLAG TIF1gamma M (456-887) was a gift from Joan Massague (Addgene plasmid # 15735 ; http://n2t.net/addgene:15735 ; RRID:Addgene_15735) -
For your References section:
Hematopoiesis controlled by distinct TIF1gamma and Smad4 branches of the TGFbeta pathway. He W, Dorn DC, Erdjument-Bromage H, Tempst P, Moore MA, Massague J. Cell. 2006 Jun 2. 125(5):929-41. 10.1016/j.cell.2006.03.045 PubMed 16751102
Map uploaded by the depositor.
