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pHCMV-LCMV-Arm53b
(Plasmid #15796)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 15796 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 15796-D50 50 μg of DNA in Tris buffer $495
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DNA 15796-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHCMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4828
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LCMVArm53b envelope glycoprotein
  • Species
    Lymphocytic choriomeningitis virus Armstrong 53b
  • Insert Size (bp)
    1497
  • GenBank ID
    AY847350

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Gene was cloned by PCR from DNA isolated from cells infected with virus obtained from The Scripps Research Institute, La Jolla, CA

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHCMV-LCMV-Arm53b was a gift from Miguel Sena-Esteves (Addgene plasmid # 15796 ; http://n2t.net/addgene:15796 ; RRID:Addgene_15796)

  • For your References section:

    Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136
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