PurposeExpression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||159423||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
/ Fusion Protein
- c-Myc and poly-His (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
- 5′ sequencing primer pBAD Forward
- 3′ sequencing primer pBAD Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
Full sequence is provided but the depositors have only verified the sequence of the multi-cloning site and the TGP-encoding region by DNA sequencing.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSB_init_Sb68 was a gift from Dianfan Li (Addgene plasmid # 159423 ; http://n2t.net/addgene:159423 ; RRID:Addgene_159423)
For your References section:An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification. Cai H, Yao H, Li T, Hutter CAJ, Li Y, Tang Y, Seeger MA, Li D. Commun Biol. 2020 Dec 10;3(1):753. doi: 10.1038/s42003-020-01478-z. 10.1038/s42003-020-01478-z PubMed 33303987