R2x2_VAL88
(Plasmid #160761)

• Purpose: Expresses R2x2_VAL88 in bacterial cells
• Depositing Lab: Nobuyasu Koga
• Publication: Koga et al Proc Natl Acad Sci U S A. 2020 Nov 23. pii: 2002120117. doi: 10.1073/pnas.2002120117.

Backbone

• Vector backbone: pET21b(+)
• Backbone size w/o insert (bp): 5358
• Total vector size (bp): 5640
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: For protein expression, BL21 star is recommended
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: R2x2_VAL88
• Alt name: Rsmn2x2_5_6_10mut
• Species: Synthetic
• Insert Size (bp): 282
• Mutation: 10-residue mutations of Rsmn2x2_5_6
• Tag / Fusion Protein:
  • 6xHis (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: David Baker, University of Washington, HHMI

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry