Sticky-351c-3X
(Plasmid
#161931)
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Purpose(Empty Backbone) DNA fragment production for dinucleosome preparation
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 161931 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUC19
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Backbone manufacturerGenscript
- Backbone size (bp) 2710
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Modifications to backboneCloned into the BamHI and HindIII sites of the pUC19 vector
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Vector typeBacterial vector for DNA production
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13 fwd
- 3′ sequencing primer M13 rev (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please cite the following article in any publications where this construct is utilized:
doi: 10.1093/nar/gkab070
Z. Adhireksan, D. Sharma, P.L. Lee, Q. Bao, S. Padavattan, W.K. Shum, G.E. Davey & C.A. Davey. (2021). Engineering Nucleosomes for Generating Diverse Chromatin Assemblies. Nucleic Acids Res.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Sticky-351c-3X was a gift from Curt Davey (Addgene plasmid # 161931 ; http://n2t.net/addgene:161931 ; RRID:Addgene_161931) -
For your References section:
Engineering nucleosomes for generating diverse chromatin assemblies. Adhireksan Z, Sharma D, Lee PL, Bao Q, Padavattan S, Shum WK, Davey GE, Davey CA. Nucleic Acids Res. 2021 Feb 15. pii: 6137298. doi: 10.1093/nar/gkab070. 10.1093/nar/gkab070 PubMed 33590100
