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(Plasmid #168877)


Item Catalog # Description Quantity Price (USD)
Plasmid 168877 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Gateway-compatible Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
  • Insert Size (bp)
  • Entrez Gene
    ORF3a (a.k.a. GU280_gp03)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site SfiI (unknown if destroyed)
  • 5′ sequencing primer M13 uni (-21)
  • 3′ sequencing primer M13 rev (-29)
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Synthesized by Twist Bioscience

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5'-linker: SfiI-BamHI-ATG-BamHI (GGCCGTCAaggccaggatccatgggatcc), 3'-linker: PacI-TAA-AsiSI-SfiI (cttaatTAAgacgcgatcgcggcctcatGGGCC).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEntry_SARS-CoV-2-ORF3a was a gift from Pascal Falter-Braun (Addgene plasmid # 168877 ; ; RRID:Addgene_168877)
  • For your References section:

    A resource of human coronavirus protein-coding sequences in a flexible, multipurpose Gateway Entry clone collection. Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, Falter-Braun P. G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 10.1093/g3journal/jkad105. 10.1093/g3journal/jkad105 PubMed 37267226