pSPLp10
(Plasmid #170462)

• Purpose: EarlyBac insect cell expression system
• Depositing Lab: Hiro Furukawa
• Publication: Chou et al Cell. 2020 Jul 23;182(2):357-371.e13. doi: 10.1016/j.cell.2020.05.052. Epub 2020 Jun 30.

Backbone

• Vector backbone: pSPL
• Total vector size (bp): 4715
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: New vector
• Promoter: dHsp70

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: multiple cloning site (unknown if destroyed)
• 3′ cloning site: multiple cloning site (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: n.a.

Resource Information

• Supplemental Documents:
  • pSPLp10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

For a more detailed description, go to:

Furukawa, H, Simorowski, N, Michalski, K (January 2021) Effective production of oligomeric membrane proteins by EarlyBac-insect cell system. In: Methods in Enzymology. Elsevier.
DOI: 10.1016/bs.mie.2020.12.019

For online protocol, go to: http://furukawalab.labsites.cshl.edu/protocols/earlybac/